Journal: Molecular Therapy Oncology

Article Title: Targeted attack: Harnessing myelin-specific plasmolipin for suppression of neuroblastoma and glioblastoma

doi: 10.1016/j.omton.2026.201154

Figure Lengend Snippet: McERV-PTLVs specifically transduce cells of brain tumors induced by GSCs injection, leaving normal brain tissues untouched (A) Mice with intracranial tumor induced by GSCs were transduced with concentrated McERV-PTLVs encoding marker EGFP. Seven days after transduction, the brain was isolated, and first cut in coronal section by the injection holes was performed. (B) Fluorescence microscopy images representing the area of the first cut demonstrate the distribution of transduced cells expressing marker EGFP. Magnified images with signed white scale bar provided. White doted lines represent the EGFP-positive area. The small image at the left upper corner of the first picture is the coronal slice stained immunohistochemically with anti-nestin antibodies representing the tumor engraftment. (C) Control group of mice was injected with 1×PBS instead of GSCs and then with McERV-PTLVs. The brain of control mice was analyzed as written above. (D) Fluorescence microscopy images of the area of the first cut represent the absence of EGFP-positive cells. The scheme of the coronal section of caudate-putamen area, where injection holes were performed (marked with red-dotted square). The small image left from the first picture is the coronal slice stained immunohistochemically with anti-nestin antibodies representing the absence of tumor. (E) Mice with intracranial tumor induced by GSCs were transduced with concentrated VSVG-PTLVs encoding marker EGFP. Black-dotted arrow below represents the timescale. (F) The fluorescence microscopy images represent the more disseminated areas of EGFP-positive cells provided on several magnifications (pointed by white arrows). The small image at the left bottom corner of the first picture is the coronal slice stained immunohistochemically, with anti-nestin antibodies representing the tumor engraftment. (G) Images of hematoxylin/eosin staining (right column) and immunohistochemical staining of slices with anti-(human) nestin antibody. Black dotted line represents the area of tumor growth. The upper row samples obtained from mice injected with GSCs and McERV-PTLVs; middle row:1×PBS and McERV-PTLVs; bottom row: GSCs and VSVG-PTLVs. Black scale bars, 100 μm. (H) Fluorescence microscopy images of IF-stained slices obtained from mice with GSC-induced tumor and injected with McERV-PTLVs. DAPI: nuclei staining (blue); anti-(human) nestin mAb stained with Alexa 555 (Red) fluor-conjugated secondary Ab, EGFP: marker fluorescent protein indicates the cells transduced with McERV-PTLVs (the merged image of EGFP [green] and anti-nestin [red] pictures are represented). The white dotted line represents the nestin-positive area of tumor growth. (I) Fluorescence microscopy images of IF-stained slices obtained from mice of control group injected with 1×PBS and McERV PTLVs, representing the absence of nestin and EGFP-positive cells. (J) Fluorescence microscopy images of IF-stained slices obtained from mice with GSC-induced tumor and injected with VSVG-PTLVs. White scale bars on H, I, and J: 100 μm.

Article Snippet: For immunohistochemical assessments, an antibody specific to human nestin (R&D Systems GmbH, Wiesbaden-Nordenstadt, Germany) was used.

Techniques: Injection, Transduction, Marker, Isolation, Fluorescence, Microscopy, Expressing, Staining, Control, Immunohistochemical staining